DETECCIÓN DE ANTICUERPOS CONTRA Lawsonia intracellularis EN PORCINOS PROVENIENTES DE GRANJAS TECNIFICADAS DEL VALLE DE LIMA Y HUARAL

El presente estudio tuvo como objetivo detectar la presencia de anticuerpos contra Lawsonia intracellularis en cerdos de granjas tecnificadas de los valles de Lima y Huaral. Los anticuerpos fueron detectados mediante la prueba de inmunofluorescencia indirecta en muestras de suero de 197 cerdos de tres edades diferentes. El 38.7% de los sueros analizados resultaron positivos, correspondiendo el 18.9, 44.3 y 45.5% a las granjas ubicadas en las zonas de Huaraz, Cieneguilla y Lurín, respectivamente. La prevalencia registrada en animales destetados, de engorde y en hembras de reemplazo fue de 24.6, 47.6 y 44.7%, respectivamente. La presencia de anticuerpos evidencia la exposición de los cerdos a la bacteria, por lo que se debe reconocer a la Lawsonia intracellularis como un nuevo agente etiológico de diarreas que afecta la industria porcina en el Perú.The aim of the present study was to detect antibodies against Lawsonia intracellularis in pigs from well-managed farms in Lima and Huaral valleys. Antibodies were detected using the indirect immunofluorescence test in 197 serum samples from pigs of three different ages. 38.7% of the samples were positive, corresponding 18.9, 44.3 and 45.5% to farms located in the areas of Huaraz, Cieneguilla and Lurín, respectively. The prevalence recorded in weaned and fattened pigs, and gilts were 24.6, 47.6 y 44.7%, respectively. The presence of antibodies indicates that these pigs have been exposed to the bacteria, so Lawsonia intracellularis should be considered as a new microorganism causing diarrhea and therefore, affecting the pig industry in the countr

EVALUACIÓN BACTERIOLÓGICA DE SEMEN DE VERRACOS USADOS COMO REPRODUCTORES EN GRANJAS PORCINAS DE LA ZONA DE LURÍN, LIMA

El uso de la inseminación artificial (IA) se ha incrementado en la crianza porcina contribuyendo al mejoramiento genético de los planteles de reproductores. Además, entre sus beneficios se encuentran la disminución de enfermedades por transmisión sexual y el menor número de reproductores requeridos; sin embargo, la difusión de esta tecnología depende en alguna forma de la calidad del semen colectado y procesado. Existen muchos factores que pueden influir directamente sobre la calidad del semen porcino (García et al., 1998), entre los cuales se encuentra la contaminación por microorganismos. La contaminación bacteriana del semen ocurre habitualmente durante la colecta (Arauz et al., 2000) o puede provenir del aparato génito-urinario, pudiendo causar una disminución de la eficiencia reproductiva (Serrano et al., 1994).The objective of the present study was to evaluate the bacterial content in semen from 30 healthy boars used as sires in pig farms in the coastal area of southern Lima (Lurín). Half of boars were from two technically advanced farms and the other half from one semi technically oriented farm. The gloved-hand method for semen collection was used and the collected semen was filtered to separate the gel fraction. The results indicated that in the technical farms, 73% of ejaculates (11/15) showed bacterial growth and 13% (2/ 15) exceeded the OIE limit of 5,013 UFC/ml, whereas in the non-technical farm , all ejaculates showed bacterial growth and 60% (9/15) exceeded the OIE limit. Pseudomona aeruginosa was the bacteria with higher number of colonies per semen sample and Escherichia coli was the most common bacteria

A los mejores estudiantes no les gusta la educación física durante la adolescencia ¿Mito o realidad? Los estudios Avena, Afinos y Up&Down

Objective: to examine if those adolescents who dislike physical education classes get better results on academic and cognitive performance than their peers. Methods: participants included 4 226 adolescents from the AVENA, AFINOS and UP&DOWN studies. Physical education enjoyment was assessed with a 7-point Likert scale. Cognitive performance in the AVENA study was assessed using the Spanish version of the SRA Test of Educational Ability. Academic performance in the AFINOS and UP&DOWN studies was assessed through Mathematics and Language grades and the average of both subjects. Results: in the AVENA study we found differences in verbal ability among girls who dislike physical education and their peers (P = 0.033). In the AFINOS study, boys who dislike physical education had higher scores in Language than their peers (P = 0.024). In the UP&DOWN study girls who disliked physical education had higher scores in Language and in the average of Language and Mathematics than their peers (P < 0.001). Conclusion: in the AVENA and AFINOS studies adolescents who disliked physical education had similar results in cognitive and academic performance than their peers, but in the UP&DOWN study girls who disliked physical education showed higher results in academic performance than their peers.Objetivo: conocer si a aquellos adolescentes que no les gusta la educación física obtienen mejores resultados en rendimiento académico y cognitivo que sus compañeros. Métodos: los participantes incluyen 4.226 adolescentes de los estudios AVENA, AFINOS y UP&DOWN. El gusto por la educación física se valoró con una escala Likert de 7 puntos. El rendimiento cognitivo se valoró en el estudio AVENA usando la versión española del SRA Test of Educational Ability. El rendimiento académico se valoró en los estudios AFINOS y UP&DOWN con las notas de Matemáticas, Lengua y la media de Lengua y Matemáticas. Resultados: en el estudio AVENA encontramos diferencias en la habilidad verbal entre las chicas a las que no les gustaba la educación física y sus compañeros (P = 0,033). En el estudio AFINOS los chicos a los que no les gustaba la educación física tenían mejores notas en Lengua que sus compañeros (P = 0,024). En el estudio UP&DOWN las chicas a las que no les gustaba la educación física obtuvieron mejores resultados en Lengua y en la media de Lengua y Matemáticas (P < 0,001). Conclusion: en los estudios AVENA y AFINOS los adolescentes a los que no les gusta la educación física obtuvieron resultados similares a los que sus compañeros, mientras que en el estudio UP&DOWN las chicas a las que no les gustaba la educación física obtuvieron mejores resultados en rendimiento académico que sus compañeros

Electronic structure analysis of the quasi-one-dimensional oxide Sr6Co5O15 within the LDA+U method

The quasi-one-dimensional cobalt oxide Sr6Co5O15 is studied using first-principles electronic-structure calculations and Boltzmann transport theory. We have been able to describe the electronic structure, characterized by the structural one-dimensionality and a particular type of charge ordering, with unexpected electronic structure of the different Co atoms. The origin of the large unquenched misaligned orbital angular momenta comes out naturally from a correct description of the different crystal-field environments. The evolution with the on-site Coulomb repulsion (U) of the electronic structure and the transport properties is discussed, with a best agreement with experiment found for the smallest value of U that allows to converge the correct in-chain ferrimagnetic ground stateThe authors thank the CESGA for the computing facilities, the Ministerio de Educación y Ciencia (MEC) for the financial support through the project MAT2009-08165, the Ministerio de Ciencia e Innovación (MICINN) for the project MAT2007-60536 and the Xunta de Galicia for the project INCITE08PXIB236052PR. A.S.B. thanks MEC for a FPU grant. M.P. and J.B. thank Isabel Barreto program and Deputación da Coruña, respectively, for financial supportS

DETECCIÓN DE ILEITIS NECRÓTICA CAUSADA POR Lawsonia intracellularis EN PORCINOS DE GRANJAS TECNIFICADAS DE LA ZONA DE LIMA

The objective of the present study was to determine the presence of Lawsonia intracellularis in Peru. A total of 197 serum samples were evaluated by the immunofluorescence test. The seroprevalence was 38.7% (73/197) and most of the affected animals were in the fattening stage. Eight percent of animals showed diarrhea and lost of body condition. Slaughtered animals showed various anatomical ileal damages. The histopathological lesions in ileon were compatible with necrotic ileitis caused by Lawsonia intracellularis. The Warthin Starry technique was used to confirm these results.The objective of the present study was to determine the presence of Lawsonia intracellularis in Peru. A total of 197 serum samples were evaluated by the immunofluorescence test. The seroprevalence was 38.7% (73/197) and most of the affected animals were in the fattening stage. Eight percent of animals showed diarrhea and lost of body condition. Slaughtered animals showed various anatomical ileal damages. The histopathological lesions in ileon were compatible with necrotic ileitis caused by Lawsonia intracellularis. The Warthin Starry technique was used to confirm these results

Moringa oleifera leaf supplementation as a glycemic control strategy in subjects with prediabetes

This article belongs to the Section Phytochemicals and Human Health.Moringa oleifera (MO) is a multipurpose plant with a high polyphenol content, which is being increasingly consumed to lessen the risk of chronic metabolic diseases such as Type 2 diabetes; however, scientific evidence from clinical trials is scarce. A double-blind, randomized, placebo-controlled, parallel group intervention study with MO leaves as a food supplement was conducted in subjects with prediabetes. They consumed six daily capsules of MO dry leaf powder (2400 mg/day) (MO, n = 31) or placebo (PLC, n = 34) over 12 weeks. Glycemia, appetite-controlling hormones and gut microbiota composition were studied. ANCOVA with the fixed factor “treatment” and the basal value as covariate was used to compare the change score between the groups. The results showed significant differences between groups in the rate of change of fasting blood glucose (FBG) and glycated hemoglobin (HbA1c), which showed opposite directions during the intervention, decreasing in MO and increasing in PLC. No different change scores were found between the groups in microbiota, hepatic and renal function markers or the appetite-controlling hormones measured. In conclusion, MO supplementation resulted in favorable changes in glycaemia markers compared to placebo in the subjects with prediabetes studied, suggesting that MO might act as a natural antihyperglycemic agent.This article is part of the R + D + i Project AGL2017-86044-C2-1-R, funded by CIN/AEI/10.13039/501100011033/ and “ERDF A way of making Europe”.Peer reviewe

A Novel Noninvasive Method Based on Salivary Inflammatory Biomarkers for the Screening of Celiac Disease

This study was supported by a grant from theSpanish Ministry of Science, Universities andInnovation (SAF2017-91873-EXP), a grant fromthe Department of Health from the BasqueGovernment (EJ-2017111082), and a researchfellowship from the Asociación de Celiacos ySensibles al Gluten de Madrid (A.C.R.). Alsosupported by a predoctoral fellowship from theUniversity of the Basque Country (M.S.dlC.) andthe Basque Government (A.O.G.)

Socioeconomic patterns in the use of public and private health services and equity in health care

<p>Abstract</p> <p>Background</p> <p>Several studies in wealthy countries suggest that utilization of GP and hospital services, after adjusting for health care need, is equitable or pro-poor, whereas specialist care tends to favour the better off. Horizontal equity in these studies has not been evaluated appropriately, since the use of healthcare services is analysed without distinguishing between public and private services. The purpose of this study is to estimate the relation between socioeconomic position and health services use to determine whether the findings are compatible with the attainment of horizontal equity: equal use of public healthcare services for equal need.</p> <p>Methods</p> <p>Data from a sample of 18,837 Spanish subjects were analysed to calculate the percentage of use of public and private general practitioner (GP), specialist and hospital care according to three indicators of socioeconomic position: educational level, social class and income. The percentage ratio was used to estimate the magnitude of the relation between each measure of socioeconomic position and the use of each health service.</p> <p>Results</p> <p>After adjusting for age, sex and number of chronic diseases, a gradient was observed in the magnitude of the percentage ratio for public GP visits and hospitalisation: persons in the lowest socioeconomic position were 61–88% more likely to visit public GPs and 39–57% more likely to use public hospitalisation than those in the highest socioeconomic position. In general, the percentage ratio did not show significant socioeconomic differences in the use of public sector specialists. The magnitude of the percentage ratio in the use of the three private services also showed a socioeconomic gradient, but in exactly the opposite direction of the gradient observed in the public services.</p> <p>Conclusion</p> <p>These findings show inequity in GP visits and hospitalisations, favouring the lower socioeconomic groups, and equity in the use of the specialist physician. These inequities could represent an overuse of public healthcare services or could be due to the fact that persons in high socioeconomic positions choose to use private health services.</p

Immune Response to SARS-CoV-2 Third Vaccine in Patients With Rheumatoid Arthritis Who Had No Seroconversion After Primary 2-Dose Regimen With Inactivated or Vector-Based Vaccines

Objective. The aim of this study was to assess the immune response after a third dose of SARS-CoV-2 vaccine in patients with rheumatoid arthritis (RA) with undetectable antibody titers after the primary regimen of 2 doses. Methods. Patients with RA with no seroconversion after 2 doses of SARS-CoV-2 vaccine and who received a third dose of either an mRNA or vector-based vaccine were included. Anti-SARS-CoV-2 IgG antibodies, neutralizing activity, and T cell responses were assessed after the third dose. Results. A total of 21 nonresponder patients were included. At the time of vaccination, 29% were receiving glucocorticoids and 85% biologic disease-modifying antirheumatic drugs (including 6 taking abatacept [ABA] and 4 taking rituximab [RTX]). The majority (95%) received the BNT162b2 vaccine and only one of them received the ChAdOx1 nCoV-19 vaccine. After the third dose, 91% of the patients presented detectable anti-SARS-CoV-2 IgG and 76% showed neutralizing activity. Compared to other treatments, ABA and RTX were associated with the absence of neutralizing activity in 4 out of 5 (80%) patients and lower titers of neutralizing antibodies (median 3, IQR 0-20 vs 8, IQR 4-128; P = 0.20). Specific T cell response was detected in 41% of all patients after the second dose, increasing to 71% after the third dose. The use of ABA was associated with a lower frequency of T cell response (33% vs 87%, P = 0.03). Conclusion. In this RA cohort, 91% of patients who failed to seroconvert after 2 doses of SARS-CoV-2 vaccine presented detectable anti-SARS-CoV-2 IgG after a third dose. The use of ABA was associated with a lower frequency of specific T cell response.Fil: Isnardi, Carolina A.. No especifíca;Fil: Cerda, Osvaldo L.. No especifíca;Fil: Landi, Margarita. Austral University Hospital; LiberiaFil: Cruces, Leonel Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Schneeberger, Emilce E.. No especifíca;Fil: Montoro, Claudia Calle. Austral University Hospital; LiberiaFil: Alfaro, María Agustina. No especifíca;Fil: Roldán, Brian M.. No especifíca;Fil: Gómez Vara, Andrea B.. No especifíca;Fil: Giorgis, Pamela. No especifíca;Fil: Ezquer, Roberto Alejandro. No especifíca;Fil: Crespo Rocha, María G. No especifíca;Fil: Reyes Gómez, Camila R.. No especifíca;Fil: de Los Ángeles Correa, Mária. No especifíca;Fil: Rosemffet, Marcos G.. No especifíca;Fil: Abarza, Virginia Carrizo. No especifíca;Fil: Pellet, Santiago Catalan. Austral University Hospital; LiberiaFil: Perandones, Miguel. No especifíca;Fil: Reimundes, Cecilia. Austral University Hospital; LiberiaFil: Longueira, Yesica Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Turk, Gabriela Julia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Quiroga, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Laufer, Natalia Lorna. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Quintana, Rosana Maris. No especifíca;Fil: de la Vega, María Celina. No especifíca;Fil: Kreplak, Nicolás. No especifíca;Fil: Pifano, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Maid, Pablo. Austral University Hospital; LiberiaFil: Pons Estel, Guillermo J.. No especifíca;Fil: Citera, Gustavo. No especifíca

Clinical intervals and diagnostic characteristics in a cohort of prostate cancer patients in Spain: a multicentre observational study

Background: Little is known about the healthcare process for patients with prostate cancer, mainly because hospital-based data are not routinely published. The main objective of this study was to determine the clinical characteristics of prostate cancer patients, the diagnostic process and the factors that might influence intervals from consultation to diagnosis and from diagnosis to treatment. Methods: We conducted a multicentre, cohort study in seven hospitals in Spain. Patients' characteristics and diagnostic and therapeutic variables were obtained from hospital records and patients' structured interviews from October 2010 to September 2011. We used a multilevel logistic regression model to examine the association between patient care intervals and various variables influencing these intervals (age, BMI, educational level, ECOG, first specialist consultation, tumour stage, PSA, Gleason score, and presence of symptoms) and calculated the odds ratio (OR) and the interquartile range (IQR). To estimate the random inter-hospital variability, we used the median odds ratio (MOR). Results: 470 patients with prostate cancer were included. Mean age was 67.8 (SD: 7.6) years and 75.4 % were physically active. Tumour size was classified as T1 in 41.0 % and as T2 in 40 % of patients, their median Gleason score was 6.0 (IQR:1.0), and 36.1 % had low risk cancer according to the D'Amico classification. The median interval between first consultation and diagnosis was 89 days (IQR:123.5) with no statistically significant variability between centres. Presence of symptoms was associated with a significantly longer interval between first consultation and diagnosis than no symptoms (OR:1.93, 95%CI 1.29-2.89). The median time between diagnosis and first treatment (therapeutic interval) was 75.0 days (IQR:78.0) and significant variability between centres was found (MOR:2.16, 95%CI 1.45-4.87). This interval was shorter in patients with a high PSA value (p = 0.012) and a high Gleason score (p = 0.026). Conclusions: Most incident prostate cancer patients in Spain are diagnosed at an early stage of an adenocarcinoma. The period to complete the diagnostic process is approximately three months whereas the therapeutic intervals vary among centres and are shorter for patients with a worse prognosis. The presence of prostatic symptoms, PSA level, and Gleason score influence all the clinical intervals differently